Table 2.
List of liver metabolites of interest in the discrimination of HPLC-ESI-HRMS-based lipidomic profiles on in both the positive and negative ionization mode and presenting a significant difference (t test on features mean, expressed as % of lipidomic profiles, with significant p-value < 0.05) between the citrulline and alanine group at PND90.
| Lipid Class | Mode | Marker | Adduct | Citrulline Mean | Alanine Mean | p-Value—Unpaired t Test with Welch’s Correction |
|---|---|---|---|---|---|---|
| Phosphatidylcholines | ESI+ | PC(32:0) PC(16:0/16:0) | (M + H)+ | 2.885 | 3.266 | 0.0444 |
| ESI+ | PC(34:1) PC(16:0/18:1) | (M + H)+ | 4.8434 | 5.3756 | 0.0137 | |
| ESI+ | PC(38:5) PC(18:2/20:3) | (M + H)+ | 0.0574 | 0.0342 | 0.0227 | |
| Phosphatidylethanolamines | ESI− | PE(36:2) PE(18:0/18:2) | (M – H)− | 3.6840 | 0.0000 | <0.0001 |
| ESI− | PE(36:4) PE(16:0/20:4) | (M − H)− | 11.4000 | 0.0000 | <0.0001 | |
| ESI− | PE(36:4) PE(18:1/18:3) | (M − H)− | 1.8010 | 0.0000 | <0.0001 | |
| ESI− | PE(38:4) PE(18:0/20:4) | (M − H)− | 13.6400 | 0.0000 | <0.0001 | |
| ESI+ | PE (38:5) PE(18:1/20:4) | (M + H)+ | 1.3860 | 1.5560 | 0.0098 | |
| ESI− | PE(38:6) PE(18:2/20:4) | (M − H)− | 0.5036 | 0.0000 | <0.0001 | |
| ESI+ | PE(38:6) PE(16:0/22:6) | (M + H)+ | 0.8097 | 0.8858 | 0.0575 | |
| ESI+ | PE(40:6) PE(18:0/22:6) | (M + H)+ | 0.4043 | 0.4734 | 0.0043 | |
| ESI+ | PE(40:6) PE(20:2/20:4) | (M + H)+ | 0.0136 | 0.0202 | 0.0085 | |
| ESI+ | PE(40:7) PE(18:1/22:6) | (M + H)+ | 0.1834 | 0.2133 | 0.0624 | |
| Phosphatidylinositol | ESI− | PI(34:2) PI(16:0/18:2) | (M − H)− | 1.4680 | 0.0000 | <0.0001 |
| Phosphatidylserine | ESI− | PS(38:4) PS(18:0/20:4) | (M − H)− | 5.8520 | 0.0000 | <0.0001 |
| Sphingomyeline | ESI+ | SM(d18:1/24:1) | (M + H)+ | 2.6510 | 3.0650 | 0.0075 |
| Ceramides | ESI− | Cer(d(18:1/20:0) | (M + HC − O2)− | 0.2765 | 1.670 | <0.0001 |