Figure 1.

Effect of genistein on kidney injury following I/R injury. In the sham group, mice received the same volume of vehicle without I/R; in the I/R group, mice received the same volume of vehicle with I/R; in the G5 group, mice were pre-treated with 5 mg/kg genistein following I/R; in the G10 group, mice were pre-treated with 10 mg/kg genistein following I/R; and in the G15 group, mice were treated with 15 mg/kg genistein following I/R. The mice were decapitated after 24 h of reperfusion for further analysis. The data were expressed as the mean ± SEM; n = 6; * p < 0.05 vs. Sham; ^# p < 0.05 vs. I/R. (a) The Scr levels and BUN levels were examined 24 h after surgery; (b) A semi-quantitative assessment of the lesion was performed by a pathologist in a blinded manner according to the ATN–scoring system. Each tubular segment visible in the cortex and the outer medulla was evaluated; (c) Representative images of the cortex and outer medulla in different groups stained with H&E. Original magnification, 400×. The black arrows indicate the areas of I/R-induced tissue damages.