Table 1. Primers used for gene cloning and expression analysis.
| Name | Primer sequence (5′ → 3′) | Application |
|---|---|---|
| 3′-CDS | AAGCAGTGGTATCAACGCAGAGTAC(T)30VN | reverse transcription |
| PaF | CTGGTGATGCAAGTGCAGTTCAG | PCR |
| PaR | TCCTCCTTACAATGAACTGTGCCA | PCR |
| pdrg-sp1 | GGAACGAACCTCAAGCCCTTATCACAA | 3′RACE |
| pdrg-sp2 | AAAATGTAGGGGGAGAAACTGTAGAAGC | 3′RACE |
| UPX-long | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | 3′RACE |
| UPX-short | CTAATACGACTCACTATAGGGC | 3′RACE |
| NUP | AAGCAGTGGTATCAACGCAGAGT | 3′RACE |
| qpdrg -F | TGCGGCAGAGGATGTTATATCA | Real time PCR |
| qpdrg-R | CCTGTGGACTGACTGGCTAAT | Real time PCR |
| rEF-F | AAGCCAGGTATGGTTGTCAACTTT | Real time PCR |
| rEF-R | CGTGGTGCATCTCCACAGACT | Real time PCR |
| pdrg B F | CGCGGATCCATGGCAGTGTCTCCAGAACGTATC | Prokaryotic expression |
| pdrg B R | CCCAAGCTTTCTACCAAGAACTTGCTTTACAGCT | Prokaryotic expression |
| pdrg NF1 | TGCGGCAGAGGATGTTATATCA | Intron amplification |
| pdrg NR1 | CCCAAGCTTTCTACCAAGAACTTGCTTTACAGCT | Intron amplification |
| pdrg NF2 | CGCGGATCCATGGCAGTGTCTCCAGAACGTATC | Intron amplification |
| pdrg NR2 | CCCAAGCTTTCTACCAAGAACTTGCTTTACAGCT | Intron amplification |