Table 2. Primers for bisulfite sequencing PCR analysis.

Sequence	Annealing (°C)	Primer sequences (5′ → 3′)	Size (bp)	Reference/
				accession
H19 DMR1	55 °C	Outer F:AGGAGATTAGGTTTAGGGGAAT	260	(Park et al., 2011)
		R: CTACCACTCCCCTCATACCTAA
		Inner F: AGTGTTTGGGGATTTTTTTTTT
		R: CACCCCATCCCCTAAATAACCCTC
H19 DMR2	55°C	Outer F:TATGTTTAGGGGTGATAAAAGT	216	(Park et al., 2011)
		R: CCCCACTTCTACAATTCAAC
		Inner F: AGGTGTTATTTTGTTTGTTGGT
		R:ATAAAATAACCTAAAAAAACTCAA
H19 DMR3	55 °C	Outer F: GGTTTTAGGGGGATATTTTTT	208	(Park et al., 2011)
		R:TTAAAAAAACATTACTTCCATATAC
		Inner F: GATTTTTAGGTTTGTTATTATTT
		R: CAAATATTCAATAAAAAAACCC
IGF2 DMR1	55 °C	Outer F:GGAAGTTTTGTTTAGTTGGTTTTT	389	(Braunschweig et al., 2011)
		R:AAATCTAAAAACAAAAACAAAAAAC
		Inner F:GTTAGGTTTAGTGTTTAGTATTGGTTT
		R: TCCAAAACCAAACCTCTCCTAC
IGF2 DMR2	55 °C	Outer F:GTTAGGGGGGGGTTTGGTTTTTTAG	268	(Braunschweig et al., 2011)
		R: CTCCCCTTAATCCTATAAAACTTCC
		Inner F: GGTAGTATTTGAAGTTTAAGAG
		R: CTATAAAACTTCCAAACAAACC