Figure 7. Ephrin reverse signaling in abducens neurons may involve Ret and p75^NTR co-receptors.

(A–E) Sholl analysis of E11.5 Chn1^WT/WT (A) and Chn1^WT/KI (B) abducens explants cultured in 1 ng/ml GDNF (blue: Chn1^WT/WT, n = 13, green: Chn1^WT/KI, n = 15, 4 experiments) (C). (D and E) Quantification of average maximum outgrowth (D) and total outgrowth (E) from C. (F–M) Sholl analysis of E11.5 Chn1^WT/WT (F) and Chn1^WT/KI (G) abducens explants cultured in 5 ng/ml NGF (Chn1^WT/WT: n = 11, Chn1^WT/KI: n = 8, 3 experiments) (H); and Chn1^WT/WT (I) and Chn1^WT/KI (J) abducens explants cultured in 25 ng/ml NGF (Chn1^WT/WT: n = 10, Chn1^WT/KI: n = 10, 3 experiments) (K). (L and M) Quantification of average maximum outgrowth (L) and total outgrowth (M) from H and K. Scale bars: 100 μm. ^†P < 0.07, *P < 0.05, **P < 0.01, ***P < 0.001. Black cross and asterisks, comparison within Chn1^WT/WT between cues by 1-way ANOVA with Dunnett’s test; red asterisks, comparison within Chn1^WT/KI between cues by 1-way ANOVA with Dunnett’s test; blue asterisks, comparisons between Chn1^WT/WT and Chn1^WT/KI within each cue by unpaired 2-tailed t test.