Skip to main content
. 2017 Mar 27;127(5):1664–1682. doi: 10.1172/JCI88502

Figure 8. Bidirectional EphA4 signaling is critical for normal abducens development.

Figure 8

(A and B) E11.5 Epha4WT/WT IslCre/+ abducens nerve in sagittal (A) and Imaris surface-rendered transverse view (B); n = 5 embryos. (C and D) Epha4fl/fl IslCre/+ abducens nerve in sagittal (C) and transverse view (D); arrows indicate wandering within area 2; n = 5 embryos. (E and F) Epha4WT/WT Twist2Cre/+ abducens nerve in sagittal (E) and transverse views (F); n = 5 embryos. (G and H) Epha4fl/fl Twist2Cre/+ abducens nerve in sagittal (G) and transverse views (H); arrows, stalled nerve bundles after exit from area 1; n = 3 embryos. nVI, abducens nucleus; O, orbit; red, neurofilament; green, Hb9-GFP. Scale bars: 100 μm. (I–L) Number of exited abducens nerve bundles (I), abducens nerve length (J), abducens diameter at orbit (K), and wandering abducens nerve bundles (L) for indicated genotypes; *P < 0.05, **P < 0.01, ***P < 0.001, 1-way ANOVA with Tukey’s test; data represent mean ± SEM. T2Cre, Twist2Cre.