Table I.
Comparison of different methods to prepare mRNA
Method | Relative Amounts (r2) | Position dependence (3’ Bias) | ORF Length Bias |
---|---|---|---|
Dynabeads oligo(dT)25 (Life Technologies) | 0.85 | Strong | Strong |
RiboMinus Yeast Transcriptome Isolation (Life Technologies) | 0.87 | Slight | Slight |
Ribo-Zero Yeast Magnetic Gold (Epicenter) | 0.98 | Slight | None |