Fig. 3. hiPSC-CMs exhibit alterations in intracellular calcium handling after 2 hour treatment with known QT interval-prolonging TKIs.

(A) Schematic illustrating TKI treatment regimen for hiPSC-CMs before calcium imaging. (B) Raw line scans of individual hiPSC-CM calcium transients following TKI treatment at indicated clinically-relevant concentration and calcium dye treatment over multiple beats. (C) Quantification of hiPSC-CM calcium imaging parameters following 2 hour TKI treatment. N=10 cells recorded for each condition. Data presented as box-and-whisker plots showing the minimum, first quartile, median, mean, third quartile, and maximum of dataset. Student’s t-test indicates significance compared to control (*P<0.05 and **P<0.01).