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. 2017 Apr 28;12(2):02C406. doi: 10.1116/1.4982596

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© 2017 Author(s).

1934-8630/2017/12(2)/02C406/8

All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 2. — Quantification of error in the stamping process. (a) Example of a transferred multiagonist pattern. Fibrinogen (green, Alexafluor 488) was stamped at a 70% surface density coverage. An inverse pattern stamp was then used to deposit collagen (red, Alexafluor 594) at a surface density coverage of 30%, precisely aligned with the first pattern. The scale bar represents 10 μm. (b) Parameters used to quantify error in the translational (X, Y) and rotational (Θ) alignment of the two stamped agonist patterns.