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. Author manuscript; available in PMC: 2018 May 1.

Published in final edited form as: Am J Transplant. 2017 Jan 27;17(5):1193–1203. doi: 10.1111/ajt.14141

A)LtxA depletes lymphocytes in vitro in a dose dependent manner (B) and preferentially depletes T_EM at higher doses (p<0.01). C) NK cells and T cells are depleted in a dose dependent manner with increased efficacy during activation with PMA/Ionomycin (p<0.01). This susceptibility to LTxA on activated CD8 T_EMs although significant (p<0.01) was already present without stimulation, only marginally increasing afterwards. This suggests an increased relative presence of the high affinity LFA-1 on CD8 T_EMs without the need for activation. D) NK cells and T cells are rapidly depleted in vivo after administration of LtxA, with rapid reconstitution after 10 hours. Subset specific depletion of CD8+ T_EM cells in vivo parallels ex vivo findings. LFA, leukocyte function antigen; LtxA, leukotoxin A.

A)LtxA depletes lymphocytes in vitro in a dose dependent manner (B) and preferentially depletes T_EM at higher doses (p<0.01). C) NK cells and T cells are depleted in a dose dependent manner with increased efficacy during activation with PMA/Ionomycin (p<0.01). This susceptibility to LTxA on activated CD8 T_EMs although significant (p<0.01) was already present without stimulation, only marginally increasing afterwards. This suggests an increased relative presence of the high affinity LFA-1 on CD8 T_EMs without the need for activation. D) NK cells and T cells are rapidly depleted in vivo after administration of LtxA, with rapid reconstitution after 10 hours. Subset specific depletion of CD8+ T_EM cells in vivo parallels ex vivo findings. LFA, leukocyte function antigen; LtxA, leukotoxin A.