Fig. 5.

SOST is over-expressed in subchondral bone osteocytes underlying partial cartilage defect (a–c) Representative staining of serial sections of subchondral bone of cores taken from region with partial cartilage defect. a H&E staining, b SOST immunostaining, c IgG negative staining, d quantification of SOST immunoreactive osteocytes in subchondral bone from macroscopically normal cartilage (core 1), partial cartilage (core 2), full cartilage defect (core 3) and osteophyte (core 4). e High magnification of immunoreactive osteocytes from slide b. a–c ×10 and e ×20 magnification. Data presented as mean ± SEM (One-way analysis of variance, Tukey’s multiple comparison test) of positive osteocytes per µm² from 6 slides per each core from 4 femoral head biopsies. ***P < 0.001 for comparison of immunoreactive osteocytes from cores taken from partial cartilage defect (cores 2) to other cores