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. 2015 Oct 1;96(Pt 10):3049–3058. doi: 10.1099/jgv.0.000257

Fig. 5.

Fig. 5.

3CLSP activity is indispensable to MAVS cleavage. (a) HEK-293T cells were transfected with Flag-tagged MAVS expression plasmids (3 μg) along with the 3CLSP or 3CLSP mutant plasmids (0.5 μg). The cells were lysed at 32 h post-transfection and analysed by Western blotting (WB). (b) HEK-293T cells were transfected with Flag-tagged MAVS expression plasmids (3 μg) along with the 3CLSP expression plasmid or an empty vector (0.5 μg). At 24 h post-transfection, the cells were treated with MG132 (20 μM) or zVAD-FMK (20 μM) for 8 h. The cells were lysed and analysed by Western blotting.