Figure 6. UCPH₁₀₁-TBOA_TFB bound EAAT1_cryst structure.

a, The movement of HP2 (purple) partly exposes the substrate-binding pocket to the solvent and shows a molecule of TBOA_TFB (purple sticks) bound to it. UCPH₁₀₁ is also observed in this structure (pink sticks). b, The tip of HP2 moves as much as 9.5 Å in the UCPH₁₀₁-TBOA_TFB- (purple), compared to the UCPH₁₀₁-substrate bound (orange) structures, placing the carbonyl oxygen of A420 away from Na2 (green sphere). c, Omit map Fo-Fc density for the TBOA_TFB molecule is contoured at 2.3σ (black mesh), and some of the residues at Van deer Waals or H-bond distance from the compound are represented as sticks.