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. Author manuscript; available in PMC: 2017 Oct 3.
Published in final edited form as: Nat Cell Biol. 2017 Apr 3;19(5):468–479. doi: 10.1038/ncb3500

Figure 2. CUL-2LRR-1 is required for CMG helicase disassembly during S-phase in C. elegans.

Figure 2

(a-b) Embryos from GFP-sld-5 mCherry-H2B worms were exposed to the indicated RNAi and processed as in Figure 1b. Timelapse images are shown from S-phase to mid-prophase. Five embryos were examined for each treatment and all behaved equivalently. Arrows denote examples of persistence of GFP-SLD-5 on prophase chromatin and scale bars correspond to 5µm. (c-d) Embryos from homozygous GFP-psf-1 / GFP-psf-1 worms were exposed to the indicated RNAi and processed as in Figure 1e-f. Unprocessed scans of key immunoblots are shown in Supplementary Figure 8.