Table 2.
Representative differentially regulated genes in zebrafish during ovulation that are conserved between zebrafish, human and mouse.
| gene symbol (mouse) | gene title | gene symbol (zebrafish) | shared type a | log2 Fold Changeb | FDR p-value |
|---|---|---|---|---|---|
| Inflammatory response and apoptosis | |||||
| Maf | Avian musculoaponeurotic fibrosarcoma (v-maf) AS42oncogene homolog | MAF (2 OF 2) | T1 | 3.65 | 1.52E-14 |
| Pla2g4a | Phospholipase A2, group IVA | cpla2 | T1 | 3.46 | 2.43E-17 |
| Azin1 | Antizyme inhibitor 1 | azin1a | T1 | 3.38 | 2.77E-14 |
| Runx1 | Runt related transcription factor 1 | runx1 | T1 | 3.27 | 2.39E-20 |
| Ptgs2 | Prostaglandin endoperoxide synthase 2 | ptgs2a | T1 | 3.25 | 4.30E-08 |
| Zbtb16 | Zinc finger and BTB domain containing 16 | zbtb16a | T1 | 3.11 | 9.48E-18 |
| Tnfrsf21 | Tumor necrosis factor receptor superfamily, member 21 | tnfrsf21 | T1 | 2.66 | 1.47E-08 |
| Gadd45a | Growth arrest and DNA-damage-inducible 45 alpha | gadd45ab | T1 | 2.45 | 7.78E-11 |
| Mcl1 | myeloid cell leukemia sequence 1 | bcl2l10 | T1 | 2.32 | 2.35E-08 |
| Litaf | LPS-induced TN factor | litaf | T1 | 1.63 | 3.44E-05 |
| Slco2a1 | Solute carrier organic anion transporter family, member 2a1 | slco2a1 | T1 | 1.62 | 7.43E-04 |
| Hipk2 | Homeodomain interacting protein kinase 2 | hipk2 | T1 | 1.28 | 4.11E-03 |
| Nr1d2 | Nuclear receptor subfamily 1, group D, member 2 | nr1d2a | T2 | 4.11 | 9.24E-26 |
| Snai2 | Snail family zinc finger 2 | snai2 | T2 | 3.43 | 8.90E-19 |
| Clu | Clusterin | clu | T2 | 2.90 | 9.23E-08 |
| Socs3 | Suppressor of cytokine signaling 3 | socs3a | T2 | 1.42 | 1.96E-04 |
| Adam8 | A disintegrin and metallopeptidase domain 8 | adam8b | T3 | 3.55 | 7.95E-25 |
| Ptger4 | Prostaglandin E receptor 4 | ptger4b | T3 | 3.13 | 5.77E-11 |
| Csf1r | Colony stimulating factor 1 receptor | csf1ra | T3 | 2.92 | 4.13E-08 |
| Nt5e | ecto-5'-nucleotidase | nt5e | T3 | 2.37 | 1.89E-05 |
| Furin | Furin | furina | T3 | 1.84 | 1.32E-04 |
| Vascularization | |||||
| Rgs2 | Regulator of G-protein signaling 2 | rgs2 | T1 | 7.21 | 4.08E-86 |
| F3 | Coagulation factor III | f3a | T1 | 4.86 | 7.20E-30 |
| Zfand5 | Zinc finger, AN1-type domain 5 | zfand5a | T1 | 3.12 | 3.93E-16 |
| Nrp1 | Neuropilin 1 | nrp1a | T1 | 1.98 | 9.89E-05 |
| Tfpi2 | Tissue factor pathway inhibitor 2 | tfpi2 | T1 | 1.71 | 8.49E-04 |
| Sema3a | Semaphorin 3A | sema3ab | T1 | 1.15 | 7.00E-03 |
| F5 | Coagulation factor V | f5 | T2 | 6.56 | 4.92E-54 |
| Serpine1 | Serine (or cysteine) peptidase inhibitor, clade E, member 1 | serpine1 | T2 | 1.84 | 1.87E-05 |
| Apoe | Apolipoprotein E | apoea | T2 | 1.67 | 9.51E-05 |
| Ppap2b | Phosphatidic acid phosphatase type 2B | ppap2b | T3 | 3.24 | 2.22E-15 |
| Adipor2 | Adiponectin receptor 2 | adipor2 | T3 | 2.07 | 1.37E-06 |
| Cell-matrix adhesion and extracellular matrix remodeling | |||||
| Tnfaip6 | Tumor necrosis factor alpha induced protein 6 | tnfaip6 | T1 | 2.25 | 1.26E-07 |
| Adamts9 | A disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 9 | adamts9 | T1 | 5.91 | 8.44E-41 |
| Ptx3 | Pentraxin 3 | ptx3b | T1 | 4.68 | 3.45E-15 |
| Timp2 | Tissue inhibitor of metalloproteinase 2 | timp2a | T1 | 3.43 | 2.34E-21 |
| Rnd3 | Rho family GTPase 3 | rnd3b | T1 | 2.33 | 1.68E-07 |
| Ezr | Ezrin | ezra | T1 | 1.84 | 1.91E-04 |
| Cd151 | CD151 antigen | cd151 | T1 | 1.76 | 5.99E-05 |
| Mkln1 | Muskelin 1 | mkln1 | T2 | 3.51 | 7.11E-18 |
| Cldn11 | Claudin 11 | cldn11a | T2 | 2.19 | 4.65E-08 |
| Palld | Palladin | palld | T2 | 2.19 | 9.19E-07 |
| Thbs4 | Thrombospondin 4 | thbs4a | T2 | 2.05 | 3.09E-04 |
| Ctnnd1 | Catenin delta 1 | ctnnd1 | T3 | 4.20 | 2.41E-12 |
| Net1 | Neuroepithelial cell transforming gene 1 | net1 | T3 | 2.79 | 2.84E-12 |
| Dapk3 | Death-associated protein kinase 3 | dapk3 | T3 | 2.09 | 1.49E-09 |
| Lox | Lysyl oxidase | lox | T3 | 1.92 | 1.83E-04 |
| Cell cycle | |||||
| Rgcc | Regulator of cell cycle | rgcc | T1 | 1.44 | 1.21E-03 |
| Ccnb2 | Cyclin B2 | ccnb2 | T1 | −2.89 | 4.82E-08 |
| Ccna2 | Cyclin A2 | ccna2 | T1 | −2.70 | 1.00E-07 |
| Mki67 | antigen identified by monoclonal antibody Ki 67 | mki67 | T1 | −2.68 | 9.26E-13 |
| Mcm3 | Minichromosome maintenance deficient 3 | mcm3l | T1 | −2.16 | 1.45E-05 |
| Cdc6 | Cell division cycle 6 | cdc6 | T1 | −1.86 | 1.06E-05 |
| Mcm10 | Minichromosome maintenance deficient 10 | mcm10 | T1 | −1.77 | 5.31E-06 |
| Chek1 | Checkpoint kinase 1 | chek1 | T1 | −1.70 | 2.67E-04 |
| Cdc20 | Cell division cycle 20 | cdc20 | T2 | −2.88 | 2.49E-10 |
| Ccnb1 | Cyclin B1 | ccnb1 | T2 | −2.72 | 5.35E-06 |
| Chek2 | Checkpoint kinase 2 | chek2 | T2 | −2.31 | 2.65E-08 |
| Cdk1 | Cyclin-dependent kinase 1 | cdk1 | T2 | −2.29 | 4.47E-07 |
| Pcna | Proliferating cell nuclear antigen | pcna | T2 | −2.12 | 2.32E-05 |
| Cdk2 | Cyclin-dependent kinase 2 | cdk2 | T2 | −1.33 | 3.34E-03 |
| Mos | Moloney sarcoma oncogene | mos | T3 | −2.67 | 2.48E-08 |
| Hormone receptor | |||||
| Lhcgr | Luteinizing hormone/choriogonadotropin receptor | lhcgr | T3 | 1.74 | 1.19E-04 |
| Esr2 | Estrogen receptor beta | esr2b | T1 | −1.39 | 1.29E-03 |
a
T1, genes that were regulated similarly among all three species; T2, genes that were regulated similarly only between human and zebrafish; T3, genes that were regulated similarly only between mice and zebrafish.
b
Changes in gene expression were based on log2 fold of the expression in wildtype relative to those in Pgr-KO. Genes were listed by fold change within each shared type in each category.