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. 2017 Feb 8;8(14):22783–22799. doi: 10.18632/oncotarget.15199

Figure 6. miR-203 re-sensitizes TMZ resistant cells through directly targeting TS.

Figure 6

(A) CCK8 assay showed that miR-203 overexpression enhanced TMZ induced cytotoxicity; (B) Illustration of the putative predicted miR-203 binding site in the TS mRNA 3′UTR region, and the mutation within the binding site was generated; (C) miR-203 targets the wild-type but not the mutant 3′UTR of TS mRNA in U87R and U251R cells; (D) miR-203 transfection significantly suppressed TS mRNA (left panel) and protein expression level (right panel) in both TMZ cell lines; (E) TS-specific siRNAs were generated and resulted in a marked decrease in TS mRNA (left panel) and protein level (right panel); (F) TS knockdown suppressed the survival cell rate of the U87R and U251R cells compared with the control; (G) Overexpression of TS with pTS dramatically abrogated the miR-203 induced re-sensitization to TMZ treatment in U87R and U251R cells. *P < 0.05, **P < 0.01, ***P < 0.001.