Figure 5. BMSCs SN arrested leukemia cell cycle/proliferation and activated AKT and mTOR intracellular signaling.

THP1 cells were cultured with or without human BMSC SN from HS5 (BM SN HS5) for 24 hours and incubated overnight with BrdU. Cells were harvested, fixed and stained with 7-AAD and analyzed using flow cytometry. (A) THP1 cells were shown to be at the different stages of the cell cycle (G₁, S and G₂/M). (B) THP1 cells were incubated in normal growth medium (M) or human BMSC SN from HS5 (HS5) or primary BMSCs SNs from AML patients (P1-P4) for 24 hours. Cell lysates were processed for western blot analysis to detect human phosphorylated mTOR, total-mTOR, phosphorylated AKT, total AKT and β-actin (loading control). Each bar represents the mean ± SD of 3 independent experiments. ***p < 0.001 (THP-1 versus THP1 SN).