Table 2. Primers used for qRT-PCR analysis of lncRNA levels.
| Target ID | Primers | Product Length (bp) | Tm (°C) |
|---|---|---|---|
| β-actin | Forward: AGCGAGCATCCCCCAAAGTT Reverse: GGGCACGAAGGCTCATCATT |
265 | 60 |
| NAALADL2-AS2 | Forward: GAGCCAACTGGGATAAAGAA Reverse: AGGAAGGCAACTGTCACTCT |
145 | 60 |
| NONHSAT120161 | Forward: ACCTGAAACGTCTACATCCA Reverse: GTTGTTCCACTGCCAAAGAG |
116 | 60 |
| NONHSAT078790 | Forward: CGGATGAAAGTACGGGTCG Reverse: GAGCTGCCTGCAAATGGTC |
167 | 60 |
| NONHSAT102729 | Forward: AGAAGCCACAGGGAAAATCA Reverse: CTCTAGCAACACGTCGCAAA |
160 | 60 |
| XIST | Forward: GGATGTCAAAAGATCGGCCC Reverse: GTCCTCAGGTCTCACATGCT |
137 | 60 |
lncRNAs = long noncoding RNAs, qRT-PCR = quantitative real-time polymerase chain reaction. The table lists the 5 primers used for qRT-PCR.