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. 2017 Feb 21;8(14):23246–23264. doi: 10.18632/oncotarget.15574

Figure 1. AF1q stable overexpression induced a spindle shape cell phenotype in A2780 ovarian cancer cells.

Figure 1

(A) Western blot showing the expression of AF1q-GFP fusion protein in A2780 cells stably transfected with a GFP-tagged AF1q vector (AF1q-GFP clones A and B) as compared to cells transfected with GFP vector (mock) and untransfected cells (UNC). The blot was incubated with GAPDH antibody, as loading control. The star indicates a non-specific band. (B) Morphological appearances of mock and AF1q-GFP A2780 clones. (C) Western blot showing AF1q protein expression in A2780 cells stably transfected with a plasmid containing the full-length AF1q coding region (clones 8 and 9) as compared to cells transfected with an empty vector (mock) and untrasfected cells (UNC). As a control for loading, the blot was incubated with GAPDH antibody. (D) Morphological appearances of mock and AF1q overexpressing A2780 clones.