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. 2017 Feb 3;8(14):23277–23288. doi: 10.18632/oncotarget.15054

Figure 4. miR-142 is an upstream regulator of THBS4 in HCC cells.

Figure 4

A. Putative microRNAs targeting THBS4 as predicted by TargetScan. B. The prediction and correlation heat map in different dataset by miRGator 3.0. C. Relative expression of miR-142 in 30 pairs of HCC and adjacent normal samples by RT-qPCR analysis (T: tumor, AN: adjacent normal). D. Inverse correlation of THBS4 and miR-142 in a group of HCC samples based on the RT-qPCR analysis. E. HuH7 cells co-transfected with P-miR-142 with wild-type (wt) or mutant (mut) pGL3-THBS4 constructs followed by luciferase reporter assays. Data were normalized by the ratio of Firefly and Renilla luciferase activities measured at 48 h post-transfection. The bar graph shows the mean ±SD in three independent transfection experiments. *P<0.05. F. Western blotting analysis of THBS4 expression in P-miR-control and P-miR-142-transfected HuH7 cells.