Figure 6. miR-142 inhibits tumor formation of HCC cells through targeting THBS4.

A. Western blot of re-expression of THBS4 in stable transfected HuH7-p-miR-142 cells. B. Representative images of transwell in vitro Matrigel cell invasion in HuH7-p-miR-142 cells expressing THBS4. C. Representative tube formation by endothelial cells after incubation with conditioned media (CM) from HuH7-p-miR-142 cells transfected with pLenti-control or pLenti-THBS4 using the tube formation assay. D. Tumor growth of xenografts of HuH7-p-miR-control and HuH7-p- miR-142. E and F. IHC staining of THBS4 and the angiogenesis marker CD31 in tumour tissues from HuH7-p-miR-control and HuH7-p-miR-142 tumor tissues.