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. 2017 Feb 21;8(14):23289–23302. doi: 10.18632/oncotarget.15576

Figure 4. Western blot analysis was done to evaluate gene expression in HCC HTB-52 cells.

Figure 4

(A) The effects of Notch activation on certain genes (HDAC4, HES1, NR4A2, p21 and p63) via over-expressing ICN1 (200 ng, 400 ng) and ICN4 (400 ng). Activated Notch signaling decreased the expression of HDAC4, p21 and p63, and increased the expression of HES1 and NR4A2. (B) The three compounds, VPA, TSA, and DBZ, affected gene expression, with all three increasing the expression of AcH4, p21 and p63, and decreasing the expression of Notch1, HES1 and NR4A2, along with a slight decrease in HDAC4, but not in H4.