Table 2. Trabecular structural changes in vertebrae of MCP-1−/− and wild-type mice fed the AIN93G or the high-fat diet.
| AIN93GWild-type No LLC | AIN93GWild-type | AIN93GMCP-1−/− | High-fatWild-type | High-fatMCP-1−/− | Diet | p valuesGene | D × G | |
|---|---|---|---|---|---|---|---|---|
| BV/TV, % | 23.7 ± 0.9 * | 19.3 ± 1.3 | 22.1 ± 0.8 | 18.4 ± 0.5 | 20.8 ± 0.7 | 0.21 | < 0.01 | 0.79 |
| Conn.D, 1/mm3 | 267.2 ± 5.8 | 271.0 ± 8.1 | 297.1 ± 7.8 | 260.9 ± 7.0 | 283.1 ± 9.0 | 0.12 | < 0.01 | 0.80 |
| SMI | 1.0 ± 0.1 * | 1.3 ± 0.1 | 1.2 ± 0.10 | 1.5 ± 0.1 | 1.3 ± 0.1 | 0.05 | < 0.05 | 0.54 |
| Tb.N, 1/mm | 5.7 ± 0.1 | 5.6 ± 0.1 | 5.7 ± 0.1 | 5.5 ± 0.1 | 5.6 ± 0.1 | 0.19 | < 0.05 | 0.68 |
| Tb.Th, μm | 47.5 ± 1.0 * | 42.8 ± 0.9 | 44.8 ± 0.7 | 42.7 ± 0.6 | 44.1 ± 0.9 | 0.65 | < 0.05 | 0.69 |
| Tb.Sp, μm | 166.6 ± 1.7 * | 175.7 ± 2.5 | 166.0 ± 2.1 | 177.2 ± 2.1 | 172.7 ± 2.6 | 0.07 | < 0.01 | 0.25 |
Two-way ANOVA was performed to compare differences among the groups of LLC-bearing mice. A priori contrasts were performed to compare the difference between AIN93G-fed wild-type mice with or without Lewis lung carcinoma (LLC);
*p<0.01 compared to AIN93G wild-type. Values are means ± SEM (n = 14-15 per group). BV/TV: bone volume fraction; Conn.D: connectivity density; SMI: structure model index; Tb.N: trabecular number; Tb.Th: trabecular thickness; Tb.Sp: trabecular separation.