Figure 4. Spy1 activation of ERK1/2 is dependent on Ras and Raf.

Hek-293 cells were transfected (A) or infected (B–D) with constructs indicated at the top of each blot (left). (A and C) were treated with inhibitors as indicated on the panels. (A, C–D) Densitometry of relative protein levels conducted over all experiments (right). Lower right panel (C) fold change ratio of pERK:Spy1 protein levels. Error bars reflect SE between at least 3 experiments. Student's t-test was performed;*p < 0.05,**p < 0.01, ***p < 0.001.