Figure 6. Inhibition of miR-155 in EMPs influences serum cytokine profiles in aGVHD mice.

After being subjected to lethal total body irradiation, BALB/c mice were transplanted intravenously with 1 × 10⁷ BM cells and 2 × 10⁷ spleen cells isolated from C57BL/6 donors to establish aGVHD model. EMPs (60 μg) from mouse TNF-α-stimulated MAECs, TNF-α-stimulated MAECs protected by 2.5 μmol/L simvastatin, TNF-α-stimulated MAECs transfected by antagomir-155, TNF-α-stimulated MAECs transfected by antagomir-NC were given intravenously to aGVHD mice on day0 and +7d, retrospectively. Antagomir-155 and antagomir-NC of 25 mg/kg was given intravenously on +7d followed by 5 mg/kg twice weekly up to +21d. Serum was taken from recipient mice on +21d and cytokines production were measured by ELISA. Shown is the mean ± SD from three combined independent experiments. *P < 0.05, ^*P < 0.01, ^***P < 0.001. (A–B) Expressions of Th1-related cytokines including TNF-α (A) and IFN-γ (B). C-E. Th2-related cytokines including IL-4 (C), IL-6 (D) and IL-10 (E–G). Th9-, Th17-related cytokines including IL-9 (F) and IL-17A (G).