Figure 7. Inhibition of miR-155 in EMPs influences T lymphocytes differentiation in aGVHD mice.

After being subjected to lethal total body irradiation, BALB/c mice were transplanted intravenously with 1 × 10⁷ BM cells and 2 × 10⁷ spleen cells isolated from C57BL/6 donors to establish aGVHD model. EMPs (60 μg) from mouse TNF-α-stimulated MAECs, TNF-α-stimulated MAECs protected by 2.5 μmol/L simvastatin, TNF-α-stimulated MAECs transfected by antagomir-155, TNF-α-stimulated MAECs transfected by antagomir-NC were given intravenously to aGVHD mice on day0 and +7d, retrospectively. Antagomir-155 and antagomir-NC of 25 mg/kg was given intravenously on +7d followed by 5 mg/kg twice weekly up to +21d. Peripheral blood T lymphocytes subsets were assessed by flow cytometry analysis on +21d. Shown is the mean ± SD from three combined independent experiments. *P < 0.05, ^*P < 0.01, ^***P < 0.001. (A, D) percentages of CD3⁺CD4⁺ and CD3⁺CD8⁺ cells (B, E) CD4⁺CD25⁺FOXP3⁺Treg out of all CD4⁺ cells (C, F) CD4⁺IL-17A⁺Th17 cells out of all CD4⁺ cells.