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. 2017 Feb 21;8(14):23401–23413. doi: 10.18632/oncotarget.15581

Figure 3. Decreased PLOD2 expression in U87 and U251 cells inhibits migration and invasion.

Figure 3

(A) Representative images from Transwell migration assays for U87-shNC and U87-shPLOD2 or U251-shNC and U251-shPLOD2 cells under normoxic or hypoxic conditions, as indicated, after 12 h (Scale bars, 50 μm). (B) Bar graphs representing quantification of the number of migrated cells per field for cell types indicated. (C) Representative images of 3D spheroid invasion assays for U87-shNC and U87-shPLOD2 or U251-shNC and U251-shPLOD2 under normoxic or hypoxic conditions for 24 h (Scale bars, 50 μm). (D) Bar graphs representing the longest invasion distance (fold change) for the cell type indicated. Data are presented as the mean ± SD (3 individual experiments). *P < 0.05; **P < 0.01; ***P < 0.001; versus shNC groups under the same condition; Student's t test.