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. 2017 Feb 21;8(14):23401–23413. doi: 10.18632/oncotarget.15581

Figure 5. Treatment with FAK inhibitor attenuates U87 and U251 cell migration and invasion.

Figure 5

(A) Western blot for the detection of FAK-p397 levels in total protein lysates (20 μg) from U87 and U251 cells treated with FAK inhibitor TAE226 (5 μM) for 12 h under normoxic and hypoxic conditions. (B) Representative images and quantification (cell number/field) of transwell migration assays for parental U87 and U251 cells treated with TAE226 (5 μM) under normoxic and hypoxic conditions (Scale bars, 50 μm). (C) Representative images and quantification of 3D invasion assays (longest invasive distance) for U87 and U251 cells treated with TAE226 (5 μM) under normoxic and hypoxic conditions (Scale bars, 50 μm). Results are presented as the mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001, versus U87-shNC or U251-shNC controls under the same conditions; Student's t test.