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. 2017 Feb 22;8(14):23470–23478. doi: 10.18632/oncotarget.15625

Figure 2. The rs1550117 A>G variant affects the transcription factor binding affinity.

Figure 2

(A) Computational analysis predicted transcription factors for DNMT3A rs1550117 A>G variant. (B) ChIP assays using Hek293 and two NSCLC tissue samples. The presence of the SP1-binding DNMT3A promoter was verified by PCR. (C) SPR analysis comparing the binding affinity of Hek293 nuclear extracts or SP1 recombinant protein to DNA probes containing either the rs1550117 A or G alleles. (D) A luciferase construct containing either the A or G allele was co-transfected with pcDNA3.1-basic (control) or pcDNA3.1-SP1 expression plasmids in A549, PC14 and Hek293 cells.