| Cooper et al, 2015 |
IHC |
monoclonal |
Mouse monoclonal anti-PD-L1 primary antibody (Merck; clone22C3) |
PD-L1 staining intensity ≥ 50% |
| Ji et al, 2016 |
IHC |
polyclonal |
Mouse polyclonal antibodies (Abcam, Cambridge, UK, ab174838) |
cases with staining intensity ≥ 2 in more than 5% of tumor cells were considered as positive |
| Yang et al, 2014 |
IHC |
polyclonal |
Rabbit anti-PD-L1 antibody (Proteintech Group Inc. Chicago, IL, USA, 17952-1-AP) |
membranous staining was present in ≥ 5% of the cells. |
| D'Incecco et al, 2015 |
IHC |
polyclonal |
Rabbit primary antibodies PD-L1 (CD274) ab58810 (Abcam, Cambridge, UK) |
all cases with staining intensity ≥ 2 in more than 5% of tumour cells were considered as positive |
| Song et al, 2016 |
IHC |
monoclonal |
Rabbit anti-PD-L1 primary antibody (Proteintech Group Inc, Chicago, IL, USA, Catalog number: 66248-1-Ig) |
positive, when membranous staining was present in ≥ 5 % of the cells. |
| Zhang et al, 2014 |
IHC |
polyclonal |
primary anti-PD-L1 antibody (SAB2900365; Sigma-Aldrich) |
positive cut off quickscore of ≥ 3 |
| Tang et al, 2015 |
IHC |
monoclonal |
Rabbit monoclonal anti-human antibody (E1L3N™, Cell Signaling Technology, Danvers, MA) |
A 5% proportion of membrane-positive tumor cells which were defined as H-score ≥ 5 have been used as cutoff for PD-L1 positivity |
| Huynh et al, 2016 |
IHC |
monoclonal |
Monoclonal antibody (E1L3N, Cell Signaling Technology, Danvers, MA) |
Positive PD-L1 expression on tumor cells for 5% cutoff |
| Inamura et al, 2016 |
IHC |
monoclonal |
anti-PD-L1 rabbit monoclonal antibody (clone: E1L3N, Cell Signaling Technology, Danvers, MA, USA) |
A score of 5% or more was categorized as PD-L1-positive |
| Ameratunga M, et al, 2015 |
IHC |
monoclonal |
Anti-PD-L1 rabbit IgG (E1L3N) cat # 13684, Cell Signaling Technology |
PD-L1 positivity was defined as > 5% cells with membranous staining of intensity 2. Strong positivity was defined as of 50% cells with membranous staining of intensity 2. |
| Takada et al, 2016 |
IHC |
monoclonal |
Rabbit monoclonal antibody (clone SP142, Spring Bioscience, Ventana, Tucson, AZ, USA). |
Positive PD-L1 protein expression for 5% cutoff |
