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. 2017 Mar 1;8(14):23551–23563. doi: 10.18632/oncotarget.15796

Figure 5. Inhibition of the PI3K/Akt signal transduction pathway decreases NDV titers.

Figure 5

(A) CEF cells were pretreated with DMSO or LY294002 (20 μM) for 1 h, and cell viability was measured and quantified by MTT cell proliferation assay at the time points indicated. (B) CEF cells were preincubated with LY294002 (20 μM) for 1 h, infected with NDV-GM at an MOI of 1, and cell culture media supernatant aliquots were harvested at 8-h intervals until 64 hpi. Virus titers in the cell supernatant were determined in CEFs via plaque assay. Data are representative of the results of three independent experiments; *p < 0.05, **p < 0.01 (t test).