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. 2017 Mar 1;8(14):23551–23563. doi: 10.18632/oncotarget.15796

Figure 9. Pharmacological inhibition of apoptosis enhances NDV-triggered cell survival.

Figure 9

CEF cells were pretreated with ZVAD-FMK (10, 20, 30, and 40 μM) for 1 h with or without LY294002 (20 μM) prior to infection with NDV-GM at an MOI of 1. (A) At 1 hpi, cells were harvested and lysed for western blot using the pAkt (Ser 473) and total Akt antibody. (B) At 24 hpi, cell lysates were harvested and subjected to western blot with caspase 3 and PARP antibodies. (C) At 24 hpi, cell viability was measured by MTT assay; *p < .05, **p < .01 (t test). (D) At 24 hpi, the number of apoptotic cells were calculated by flow cytometry using Annexin V-FITC/PI Apoptosis Detection Kit; *p < .05 (t test).