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. 2017 Mar 1;8(14):23564–23574. doi: 10.18632/oncotarget.15797

Table 2. Sequences of oligonucleotides used as primers for real-time PCR, siRNA and ChIP-qPCR.

Construct Sequences
Primers for real-time PCR
HRD1 Sense 5′-AACCCCTGGGACAACAAG-3′
Antisense 5′-GCGAGACATGATGGCATCT-3′
S100A8 Sense 5′-ATGCCGTCTACAGGGATGAC-3′
Antisense 5′-ACTGAGGACACTCGGTCTCT-3′
GAPDH Sense 5′-ATGGGGAAGGTGAAGGTCG-3′
Antisense 5′-GGGGTCATTGATGGCAACAATA-3′
Primers for siRNA
Si S100A8 001 Sense 5′-CUAUCAUCGACGUCUACCATT-3′
Antisense 5′-UGGUAGACGUCGAUGAUAGTT-3′
Si S100A8 002 Sense 5′-GGAAUUUCCAUGCCGUCUATT-3′
Antisense 5′-UAGACGGCAUGGAAAUUCCTT-3′
Si S100A8 003 Sense 5′-GACGUCUGGUUCAAAGAGUTT-3′
Antisense 5′-ACUCUUUGAACCAGACGUCTT-3′
Primers for CHIP-qPCR
−835~−618 Sense 5′-AAGCAGGTGAGTGGTTGTTAGGG-3′
Antisense 5′-ACAAACAGAACACTTCCGTCCC-3′
−639~−428 Sense 5′-CAACAGTTTGACAAATACGGTGC-3′
Antisense 5′-CCTAACAACCACTCACCTGCTTT-3′
−401~−188 Sense 5′-GGTGGTCGGAAGGTAATAAGGC-3′
Antisense 5′-CATTTCATTCCCCTCAGGCTTGT-3′