Primers for real-time PCR |
|
|
HRD1 |
Sense |
5′-AACCCCTGGGACAACAAG-3′ |
|
Antisense |
5′-GCGAGACATGATGGCATCT-3′ |
S100A8 |
Sense |
5′-ATGCCGTCTACAGGGATGAC-3′ |
|
Antisense |
5′-ACTGAGGACACTCGGTCTCT-3′ |
GAPDH |
Sense |
5′-ATGGGGAAGGTGAAGGTCG-3′ |
|
Antisense |
5′-GGGGTCATTGATGGCAACAATA-3′ |
Primers for siRNA |
|
|
Si S100A8 001 |
Sense |
5′-CUAUCAUCGACGUCUACCATT-3′ |
|
Antisense |
5′-UGGUAGACGUCGAUGAUAGTT-3′ |
Si S100A8 002 |
Sense |
5′-GGAAUUUCCAUGCCGUCUATT-3′ |
|
Antisense |
5′-UAGACGGCAUGGAAAUUCCTT-3′ |
Si S100A8 003 |
Sense |
5′-GACGUCUGGUUCAAAGAGUTT-3′ |
|
Antisense |
5′-ACUCUUUGAACCAGACGUCTT-3′ |
Primers for CHIP-qPCR |
|
|
−835~−618 |
Sense |
5′-AAGCAGGTGAGTGGTTGTTAGGG-3′ |
|
Antisense |
5′-ACAAACAGAACACTTCCGTCCC-3′ |
−639~−428 |
Sense |
5′-CAACAGTTTGACAAATACGGTGC-3′ |
|
Antisense |
5′-CCTAACAACCACTCACCTGCTTT-3′ |
−401~−188 |
Sense |
5′-GGTGGTCGGAAGGTAATAAGGC-3′ |
|
Antisense |
5′-CATTTCATTCCCCTCAGGCTTGT-3′ |