Figure 4. Compounds 1 and 2 inhibit dUTPase activity.

Chemical structures of the dUTPase inhibitors 1 (A) and 2 (B) (C) Potency of compounds 1 and 2 was assessed by malachite green assay, using dUTP as a substrate. Percentage activity was calculated compared to DMSO treated control. Inhibition curves shown are representative curves of two independent inhibition experiments performed using duplicate measurements. IC₅₀ values displayed in (A) and (B) were determined from two independent experiments performed in duplicate and are shown as average ± SD. (D) Superposition of the top-ranked docking poses of compounds 1 (blue sticks) and 2 (orange sticks). The different monomers of the protein are rendered as green, cyan and magenta cartoons. H-bonds are shown as yellow dotted lines.