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. 2017 Feb 28;8(14):23713–23726. doi: 10.18632/oncotarget.15785

Figure 5. The dUTPase inhibitors sensitize cells to 5-FU treatment.

Figure 5

(A) Resazurin experiment assessing the viability of SW620 cells co-treated with 5-FU and the dUTPase inhibitor 2 at the indicated concentrations for 72 hours. Values were normalized against the no-DMSO control (−). Data shown as average ± SEM from three independent experiments performed in duplicate. (B) Resazurin experiment analyzing cellular viability after 72 hours of co-treatment with 5-FU and compound 2 (10 μM) in combination with thymidine (20 μM). Values were normalized against the DMSO control. Data shown as average ± SEM from at least four independent experiments performed in duplicate. (C) Viability of HeLa, TOV-112D and U2OS cells treated with 10 μM of compound 1 or 2 and increasing concentrations of 5-FU. After 72 hours of the indicated treatment cell viability was analyzed by Resazurin. Data shown as average ± SEM from at least three independent experiments performed in duplicate. (D) Western Blot analyzing the dUTPase levels in the indicated cell lines. β-Actin was used as a loading control.