Figure 3. PCDH7 and mutant EGFR cooperate to promote MAPK activation and tumor growth of NSCLC cells.

A, Western blot analysis demonstrating strong ERK activation in HBEC-shp53-EGFR^L858R cells overexpressing PCDH7. B, Quantification of soft agar assays demonstrating enhanced transformation of HBEC-shp53 cells and HBEC-shp53-EGFR^RRL858R cells with PCDH7 expression. C, Western blot demonstrating elevated ERK activation in PC9 cells overexpressing PCDH7. D, Soft agar assays and quantification in EGFR mutant PC9 cells showing that enforced expression of PCDH7 increased colony formation. E, Quantification of tumor volume in NSG mice injected with PC9 cells with and without enforced PCDH7 expression (**p<0.01 vs. control, Student's t-test).