Figure 4.
Cartoon depicting two models for how the host and E. coli compete for iron that involve enterobactin (Ent) and lipocalin-2 (LCN2). Canonical model: LCN2 sequesters Fe(III)-Ent in the extracellular space and thereby prevents Fe uptake by E. coli. Alternative model based on studies in human urine: LCN2 binds aryl metabolites present in urine and sequesters Fe(III). Metallation of Ent contributes to the demetallation of LCN2. Ent may prevent Fe(III) from binding to LCN2 and/or sequester Fe(III) from the Fe(III)-catechol-LCN2 complex, and the resulting Fe(III)-Ent provides nutrient iron to E. coli. Iron-bound LCN2 might be transported into host cells by receptor-mediated uptake. Abbreviations: CM, cell membrane; OM, outer membrane; IM, inner membrane.