Figure 2.

5-aza sensitizes cells to TGF-β1 as indicated by IL11 expression. Human gingival fibroblasts were exposed to 5-aza or left untreated for 72 hours, before cells were stimulated with recombinant human TGF-β1. After 24 hours, gene expression analysis was performed. (A) IL11 (n=10), (B) PRG4 (n=7), (C) NOX4 (n=13), with data from different experiments marked with separate colors. Data indicate the x-fold changes in response to 5-aza in untreated and TGF-β1 treated cells, respectively. P values were obtained from the paired t-test. Statistical analyses were based on fold-change values of single experiments. The colors represent data from different cell donors and experiments.

5-aza: 5-aza-2'-deoxycytidine, TGF-β1: transforming growth factor-β1, IL11: interleukin-11, PRG4: proteoglycan 4, NOX4: NADPH oxidase 4, w/o: without.