Table 5. TGF-β1 increases TGF-βRI without 5-aza and decreases TGF-βRII in the presence of 5-aza.

Cell treatment	TGF-βRI (n=14)		TGF-βRII (n=14)
	Median (min, max)	P value	Median (min, max)	P value
SF	2.14 (0.89, 7.40)	0.005	0.80 (0.27, 1.53)	0.057
5-aza	1.78 (0.45, 5.05)	0.096	0.41 (0.14, 2.89)	0.011

Human gingival fibroblasts were exposed to 5-aza or left untreated for 72 hours in SF medium, before cells were stimulated with recombinant human TGF-β1. After 24 hours, the gene expression analysis was performed. P values reflect the changes of gene expression in response to TGF-β1 compared to unstimulated cells. Statistical analyses were based on fold-change values with the paired t-test.

TGF-β1: transforming growth factor-β1, TGF-βRI: transforming growth factor-β receptor type I, 5-aza: 5-aza-2'-deoxycytidine, TGF-βRII: transforming growth factor-β receptor type II, min: minimum, max: maximum, SF: serum-free.