Table 7. 5-aza increases DNMT1 and DNMT3B, with a similar trend in the presence of TGF-β1.

	DNMT1 (n=10)		DNMT3A (n=10)		DNMT3B (n=9)
	Median (min, max)	P value	Median (min, max)	P value	Median (min, max)	P value
SF	1.61 (0.97, 3.99)	0.005	1.30 (0.23, 4.40)	0.310	1.53 (1.07, 3.84)	0.002
TGF-β1	1.41 (0.85, 3.07)	0.015	0.65 (0.52, 2.33)	0.062	1.54 (0.71, 4.25)	0.067

Human gingival fibroblasts were exposed to 5-aza or left untreated for 72 hours, before cells were stimulated with recombinant human TGF-β1 or left in serum-free medium alone. After 24 hours, the gene expression analysis was performed. P values reflect the ratio of gene expression in response to 5-aza compared to unstimulated cells. Statistical analyses were based on fold-change values with the paired t-test.

5-aza: 5-aza-2'-deoxycytidine, DNMT: DNA methyltransferase, TGF-β1: transforming growth factor-β1, min: minimum, max: maximum, SF: serum-free.