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Successful 2P photolysis optimization using pre-predetermined Zernike modes. Responses from twelve (12) L5 pyramidal neurons for: (a) astigmatism, NZT = 5 (p-value = 0.00015, diamonds) (b) coma-x, NZT = 8 (p-value = 0.00077, triangles), and (c) spherical aberration, NZT = 11 (p-value = 0.00039, squares). (d) As expected, correction with coma-y did not produce optimized responses. ***p < 0.001.
