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CD4-lineage positive cells are in close proximity to the epiphyseal growth plate. Hindlimbs from 5-week-old WTCD4.tdTom and DKOCD4.tdTom mice were cryosectioned then stained with DAPI and wheat germ agglutinin. (A) Composite widefield sections of tibia obtained at 10× were digitally stitched together. (B) Sections from (A) were electronically zoomed. (C) Confocal images of femur sections taken at 20×. Representative images of at least three mice per group are presented.
