Table 1.
Antibodies.
| Antigen | Immunogen | Manufacturer, species raised in, mono/polyclonal, Cat. no. | Dilutions |
|---|---|---|---|
| Beta-actin | A slightly modified synthetic b-cytoplasmic actin N-terminal peptide DDDIAALVIDNGSGK conjugated to KLH | Sigma, mouse monoclonal, A5441 | 1:250000-500000 Western blot |
| FLAG-M2 | DYKDDDDK FLAG epitope | Stratagene (Agilent), mouse monoclonal, 200471 | 1:2000-10000 Western blot/8 μg CoIP |
| Myc | EQKLISEEDL tag human c-Myc, AA 410-419 | Roth, rabbit polyclonal, 4667.1 | 1:2000-10000 Western blot/8 μg CoIP |
| V5 | GKPIPNPLLGLDST | Life Technologies, mouse monoclonal, R960-25 | 1:2000-10000 Western blot |
| FoxP1 | Full length native protein, purified from mouse FOXP1 | Abcam, mouse monoclonal, ab32010 | 1:2000-5000 Western blot/8 μg CoIP |
| FoxP2 | Synthetic peptide (C) REIEEEPLSEDLE corresponding to amino acids 703-715 of the C-terminus of human FOXP2 | Abcam, rabbit polyclonal, ab16046 | 1:2000-5000 Western blot/8 μg Co-IP |
| FoxP4 | The epitope recognized by A302-394A maps to a region between residue 1 and 50 of human forkheadbox P4 using the numbering given in entry NP_001012426.1. | Bethyl, rabbit polyclonal, A302-394A | 1:2000-5000 Western blot/8 μg Co-IP |