Figure 1.

Elongator interacts with connexin 43 (Cx43) in the developing cortex. (A) Scheme illustrating the location of the coronal section (12 μm) of E14.5 wild-type (WT) cortex. (B–H) Immunodetection of Cx43 (red) and Elp3 (green) in E14.5 WT cortex showing co-expression of both Elp3 and Cx43 in cortical plate (CP) neurons (C–E) and in the ventricular (VZ)/subventricular zones (SVZ) neuronal progenitors (F–H). (I–L) Immunoprecipitates from E14.5 mouse embryos cortices were subjected to anti-ELP1 or anti-Cx43 western blot analysis; corresponding western blots were performed on crude cell extracts (inputs). (M) Immunoprecipitates from HEK293 cell line stably expressing flag-ELP3 were subjected to anti-Cx43 western blot analysis and showed an interaction between Cx43 and ELP3. Corresponding western blots were performed on crude cell extracts (inputs). (N) Forty-eight hours after transfection of Cx43 in N2A cells, immunoprecipitates from N2A cells homogenate were subjected to anti-ELP1 western blot and showed an interaction between Cx43 and ELP1. Bar scale, 50 μm.