Figure 13.

Radial glial cells show little ELOVL4 labeling. (A) Many cells (^*) in the external and internal granule cell layers (egl and igl, respectively), as well as cells migrating through the molecular cell layer (mcl), of the developing cerebellum show ELOVL4 labeling at P10. (B) Radial glial cells and their processes (arrowheads) spanning the layers of the cerebellum can be identified by labeling for glutamine synthtase (GS). (C) Overlay of (A,B) shows no colocalization of labeling for ELOVL4 and GS. (D) Many cells in the developing cerebral cortex show ELOVL4 labeling (^*) at P10. (E) Radial glial cells and their processes (arrowheads), identified by labeling for glial fibrillary acidic protein (GFAP). Non-specfic labeling of a blood vessel (bv) also is visible. (F) Radial glial cells and their processes do not show labeling for ELOVL4. Nuclei counterstained with DAPI (blue) in all panels. Scale bars = 50 μm.