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. 2017 May 1;8:685. doi: 10.3389/fpls.2017.00685

FIGURE 1.

FIGURE 1

Analysis of pZm908 deletion mutants in transgenic tobacco plants. (A) Deletion constructs consisting of putative pollen-specific cis-regulatory elements as shown in Table 1. (B) Histochemical staining of GUS activity in the transgenic tobacco of pZm908 deletion constructs (a–e). (B-a) The mature pollen grain, (B-b) seedling leaves, (B-c) seedling roots, (B-d) immature seeds, (B-e) indehiscent anthers, (B-f) leaves of 35S::GUS transgenic tobacco as a positive control, (B-g) root of 35S::GUS transgenic tobacco as a positive control, and (B-h) immature seeds of p19Z::GUS transgenic tobacco as a positive control. Mature pollen grains were collected from intraday dehiscent anthers. Three independently isolated transgenic tobacco lines were analyzed for p1670, p1126, p789, p184, and p126; two lines were analyzed for p908, p586 and p244.