FIGURE 2.

Analysis of p184 promoter deletion mutants in transgenic tobacco plants. (A) The sequence of –126 to –68 region of pZm908 in Zong31. The location of the deletion constructs and putative cis-regulatory elements are indicated. (B) Homology analysis of p184 segments in Zong31, Mo17 and B73. The putative cis-regulatory elements are indicated. (C-a) Deletion constructs consisting of putative cis-regulatory elements located in p184. (C-b) Histochemical staining of GUS activity in mature pollen grains of transgenic tobacco plants. (C-c) Scatter histograms showing quantitative determination of GUS activity in mature pollen grains of transgenic tobacco plants. Four individual lines harboring each p184 promoter deletion construct were analyzed. The values represent the mean ± SD from four separate plants of each construct. Significant differences were assessed by the Student’s t-test using a P-value < 0.05.