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. 2017 May 1;9:121. doi: 10.3389/fnagi.2017.00121

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Copyright © 2017 Zhang, Zhang, Zhan, Li, Zhou, Xing, Jiang, Chen and An.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of mice behavior in Morris water maze. Morris water maze was conducted to test spatial learning and memory in a circular pool filled with milk-opacified water. The procedure included escape trials (4 days) and a probetrial (1 day). In escape trials, an escape platform (8 cm in diameter) was placed 1 cm below the water surface. Each mouse was given 4 trials with 60 s intertrial interval per day. In the probe trial, mice swam freely in the water tank without the platform for 60 s. (A) Escape latency to the hidden platform in escape trials. (B) Pass through the region of the original platform (passing times) in the probe trial. (n = 10; mean ± SD; two-way ANOVA with repeated measures for escape latency and one-way ANOVA for passing time both followed by LSD multiple comparison tests; ^*p < 0.05 and ^**p < 0.01 vs. WT group, #p < 0.05 and ^##p < 0.01 vs. WT+SFN group, Δp < 0.05 vs. AD group).