FIGURE 5.

De novo translation and transcription are required for LTM, but not for STM. Animals were cultured on agar plates containing anisomycin, cycloheximide, or actinomycin D for 4 h (massed training) or 2 h (spaced training) at RT, and were then conditioned with 0.01% 1-nonanol and 160 mM KCl by eight-cycle massed training (open bars) or eight-cycle spaced training with a 10-min ITI (gray bars). Chemotaxis assays were performed immediately after training. LI values were calculated from the equation shown in Figure 2A using CI_reference values of unpaired animals treated with 0.01% 1-nonanol and 160 mM KCl (ISI, 120 s). Asterisks (^∗∗) indicate statistically significant differences (p < 0.01) determined by one-way ANOVA, followed by the Tukey-Kramer test for further comparison with LIs of untreated animals. Bars are means ± SEM (n = 6–9 assays).