FIGURE 6.

Caenorhabditis elegans mutants defective in learning and memory. Wild-type N2 and mutants were conditioned with 0.1% 1-nonanol and 160 mM KCl using eight-cycle massed training (open bars) or eight-cycle spaced training with a 10-min ITI (gray bars). Chemotaxis assays were performed immediately after conditioning. LI values were calculated from the equation shown in Figure 2A using CI_reference values of unpaired animals treated with 0.1% 1-nonanol and 160 mM KCl (ISI, 120 s). Asterisks indicate statistically significant differences (^∗p < 0.05, ^∗∗p < 0.01) of mutant LIs in comparison with those of the wild-type, using two-sided Student’s t-test. Data are displayed as means ± SEM (n = 6–9 assays).